The Brazilian is expected to serve the second of the three game ban for his red card and subsequent sarcastic applause of the officials in 2-0 defeat to Malaga a fortnight ago.
However, the club have explored every option to have him available.
An appeal to Spain's court of Arbitration for sport (TAD) was delibeartely delayed until after the court's normal friday meetings to decide on appeals with the hope that would automatically see the ban suspended.
However, TAD could still call an emergency meeting on saturday, and even if they don't, Barca would risk being sanctioned with defeat for fielding an ineligible player should Neymar feature.
According to Luis Enrique who is leaving Barca at the end of the season, "I think the club are correct in their position to defend the club's interest".
"As a coach, i am prepared to play with or without Neymar".
Barcelona who are behind Real Marid with three points having played a game more than their title rivals, have their hopes raised high of contending for the title if they win at the Santiago Bernabeu.
Remember Barca were dumped out of the champions league by Juventus, but Luis Enrique still believes his palyers should see the chance to bounce back against Real Madrid as an opportunity and not an extra pressure.